I am planning to use Hygromycin B (HY-B0490) as an antibiotic selection marker. However, the reconstitution instruction requires ultrasonic for both DMSO and water and we do not have a sonicator. Is there an alternative approach?
I would also like to perform optimization on the concentration of Hygromycin B in cells. I am wondering if the subsequent dilutions after diluting the stock in water, can it be done in cell culture media?
Regarding the first question: you can also heat the solution in a water bath at 37-55°C to aid dissolution.
Second question: it can be diluted with cell culture medium, but it should be used directly after dilution, and storage is not recommended.
The answer to this question is provided by MedChemExpress Technical Support.
@Marine Ma No, a sonicator is not necessary when reconstituting or diluting Hygromycin B, as it dissolves readily in sterile water or buffer solution and can be dissolved by gentle agitation, heat, or simply by following the instructions provided by the manufacturer.
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