Recently I bought SB431542 (HY-10431) and SRI-011381 (HY-100347), and I am planning to administer SB431542 at a dose of 10 mg/kg and SRI-011381 at 20 mg/kg to 20 g mice every two days. Could anyone provide guidance on how to properly prepare these medications for administration? Any specific recommendations regarding dilution, solubility, or vehicle would be greatly appreciated!
Qiu Weiwei
SB431542 10 mg/kg Recommended Protocol:
- Vehicle composition: 2% DMSO + 40% PEG300 + 5% Tween-80 + 53% Saline
- For a 20 g mouse, administer 200 μL (working concentration: 1 mg/mL; DMSO stock concentration: 50 mg/mL).
SRI-011381:
- Vehicle composition: 50% PEG300 + 50% Saline (Clear solution)
- Stock concentration: 5 mg/mL (prepared via sonication)
- For a 20 mg/kg dose in a 20 g mouse, administer 200 μL (working concentration: 2 mg/mL).
- Preparation: Directly weigh the powder and prepare fresh; storage is not recommended. Example: To prepare 1 mL, weigh 2 mg powder, dissolve in 500 μL PEG300, mix thoroughly, then add 500 μL saline and mix until clear. Directly administer after preparation. Note: Sonication and gentle heating (37–40°C) may be used to aid dissolution if needed.The answer to this question comes from MedChemExpress Technical [email protected]
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