Is It Normal to Encounter Sticky Residues When Lysing Cells? How Should I Address This Issue?

30 July 2025 1 2K Report

I have observed that my cell lysates tend to be sticky during the lysis process. Is this a common occurrence? I'm using MCE NP-40 Lysis Buffer (HY-K1002). Any guidance or suggestions would be greatly appreciated!

Lucian Miles

Marine Ma , a sticky feeling after cell lysis is usually caused by DNA released from the cells. DNA itself is sticky, which can cause the lysis buffer to appear sticky. This may indicate that the cells have not been fully lysed. You can add more lysis buffer to promote complete cell lysis.

The answer to this question comes from MedChemExpress (MCE) Technical Support.

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