Meclofenamic acid fails to suppress FTO expression at 1-200 μM: Methodological insights or biological nuance?

04 August 2025 1 5K Report

I'm investigating pharmacological inhibition of FTO using meclofenamic acid sodium (MA). Despite treating the cell for 24-48h across a broad concentration range (1-200 μM), we observe no reduction in FTO levels (data attached). Any insight would be greatly appreciated!

Qiu Weiwei

This inhibitor functions as a competitive inhibitor of the interaction between FTO and m6A, rather than reducing the expression level of FTO. Therefore, the validation method is incorrect. Please refer to the following literature for reference: PMCID: PMC4288171.

The answer to this question comes from MCE Technical Support.

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