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Questions related from Chloe Sun
There are different versions of Dulaglutide with very different molecular weights so I just want to: 1) double check what molecular weight it has (HY-P0120) and 2) also if possible, get a...
4 weeks ago 4,978 1 View
Mal-PEG4-NHS ester The experimental goal is to conjugate an IgM antibody (total molecular weight 144.7 kDa) to a single-stranded DNA (20-30 kDa), which has a thiol group at one end, using this...
4 weeks ago 8,684 1 View
For subcutaneous tumor formation experiments, which one do you recommend between Basement Membrane Matrix (Phenol Red) (HY-K6001) and Basement Membrane Matrix (HY-K6002)?
4 weeks ago 5,717 1 View
I want to use the H2DCFDA probe to detect changes in ROS levels in cells after viral infection. However, since the virus cannot be taken out of the high-level laboratory for flow cytometry, I...
4 weeks ago 4,948 1 View
What is the solubility of Losartan (HY-17512) in DPBS or water?
last month 5,664 1 View
I am planning to perform animal experiments using Biochanin A with intraperitoneal administration. The required working concentration is 5 mg/mL. Could anyone share an appropriate dissolution or...
last month 8,745 2 View
I'd like to use a recombinant protein to activate B cells, but I'm unsure which one to choose. If anyone has relevant experimental experience to share, I'd be very grateful!
last month 7,862 1 View
Is there a recommended dosage for administering Butylphthalide (HY-B0647) via oral gavage in SD rat animal studies, as no suitable dosage was found in the literature?
last month 4,451 1 View
Type VII collagenase (HY-E70005J) is used to establish a rat model of intracerebral hemorrhage, with an administration concentration of 0.3 U/μL. How can the stock solution and working solution be...
last month 9,477 1 View
I want to prepare a 9 mg/ml solution of RIG012 for mouse experiments. The product manual recommends dissolving the drug in DMSO, with a drug solubility of 33 mg/ml. However, it requires the DMSO...
last month 4,856 1 View
I am planning to use carnosic acid (HY-N0644) in a rat model, specifically in weakened rats where the final DMSO concentration should be kept below 2%. Could anyone share practical dissolution or...
last month 6,484 1 View
I’m preparing LY294002 (HY-10108) for in vivo use in mice (10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline). At 50 mg/ml in DMSO, the solution isn’t fully clear, and at 5 mg/ml working...
last month 232 1 View
I would like to inject BMS-309403 (HY-101903) into mice. How should I calculate the dosage, and should I dissolve it in DMSO?
last month 7,900 1 View
BMP signaling agonist sb4 (HY-124697) What is the specific mechanism by which this small molecule compound Sb4 activates the BMP/PSMAD signaling pathway? For example, does it promote...
last month 7,999 1 View
Etomoxir (HY-50202) is a carnitine palmitoyltransferase 1a (CPT-1a) inhibitor. Perhexiline maleate (HY-B1334A) is an orally active inhibitor of both CPT1 and CPT2. Can they be dissolved using PEG8000?
last month 4,404 1 View
Herceptin protein (HY-P9907), the storage conditions in the instruction manual state that it should not be frozen. Is this referring to the powder or the reconstituted sample? Additionally, the...
last month 294 1 View
Currently, I am crosslinking biglycan onto my scaffold and need to evaluate its bioactivity post-crosslinking. I noticed that MCE has previously tested biglycan’s activity by examining its...
last month 1,739 1 View
I am interested in doing an experiment on exosome research. I want to attach a fluorescent tag to exosomes or vesicles extracted from cells, and then put the exosomes or vesicles back into another...
last month 9,212 2 View
I am using Sorafenib on Huh7 and HepG2 cells. At 150 µM in 0.5% DMSO I observed something unusual in the drug-treated cells, not seen in the DMSO control. Could this be contamination or improper...
last month 4,620 2 View
I plan to use a 50% PEG300 + 50% Saline solution as the dissolving vehicle for oral gavage administration in mice.
last month 2,197 1 View
I'm conducting a Syber Green PCR study. I used only Syber Green Master Mix and forward-reverse primers as the negative control, but the results were still not consistent. Patients, negative...
last month 7,710 3 View
I am planning to use HMGB1 (MedChemExpress, HY-P72797) to stimulate TNF-a induction in Raw246.7 cells. What would the detailed protocol be in order to achieve something similar to the ED50 curve...
last month 2,603 2 View
Animal model is a diabetic model of Apoe-/- mice (C57BL/6) fed a high-fat diet. Can the GPR132 antagonist 1 (HY-157189) be administered via intraperitoneal injection? What is the recommended dosage?
last month 2,524 1 View
Using Kurarinone for i.p. administration in mice, I have the following questions: 1. SBE-β-CD (Sulfobutylether-β-Cyclodextrin), after dissolving it in saline, how should it be stored? 2. I am...
20 August 2025 9,541 1 View
I plan to purify a protease product from mouse cell samples and subsequently characterize its activity. Is the MCE HY-K1000 kit suitable?
19 August 2025 392 4 View
DKK-1 Protein, Mouse (CHO) (HY-P7154) did not show significant inhibitory effects when used in organoids. It was found that the culture medium contains Wnt3a, R-Spondin1, and EGF, which have...
19 August 2025 9,623 1 View
I am currently conducting a research study and am interested in using enalapril as a positive control in my animal model (Wistar rats). Specifically, I am looking for a water-soluble form of...
14 August 2025 4,346 1 View
While I have reviewed several previous studies involving OGT2115, I have found considerable variation in the solvent compositions used to dissolve the compound. Therefore, I would like to inquire...
14 August 2025 6,711 1 View
What is the recommended annealing temperature for the U6 primer provided in the MicroRNA RT Master Mix for qPCR III (Poly A) kit (MedChemExpress HY-K0512)? Thank you in advance!
14 August 2025 9,207 4 View
I’m considering purchasing KB-0742 and comparing the differences between the dihydrochloride salt (HY-137478A) and the free base forms (HY-137478). Generally, the dihydrochloride salt would...
11 August 2025 1,875 2 View
I’m using a diabetic nephropathy mouse model and planning to give the drug at 20 mg/kg via IP injection. Do you think normal saline (0.9% NaCl) would work for dissolving it, or should I consider...
08 August 2025 2,997 1 View
I noticed that Tavapadon (HY-119486) was originally designed as an oral drug. However, I am wondering if there is any specific formulation for systemic administration of the drug. I am looking to...
07 August 2025 5,426 1 View
I plan to use phalloidin to label the cytoskeleton, but I'm not sure about the specific protocol. I've found standalone phalloidin as well as fluorescently conjugated phalloidin, such as...
06 August 2025 4,200 2 View
I am considering using AF 647 carboxylic acid (HY-D2160) to label CD19 protein, but I’m unsure whether it’s feasible. Thus, I’d like to confirm which functional groups AF 647 carboxylic acid can...
05 August 2025 7,726 1 View
I need to determine whether Oxonol VI is a slow-response or fast-response potential-sensitive probe in order to establish the appropriate experimental conditions and detection methods for my study.
05 August 2025 2,183 1 View
In my experiment, PC12 cells were treated with 50 μM Roxadustat for 6 hours, followed by protein extraction and Western blot detection.
01 August 2025 1,291 1 View
Observation: In my RSL3-induced ferroptosis mouse model, no significant reduction in GPX4 protein levels was observed under certain treatment conditions. Experimental Details: RSL3...
30 July 2025 8,478 1 View
Hi everyone, While performing a cell experiment, I noticed that precipitation occurs when I dilute the stock solution of Clozapine N-oxide (CNO, HY-17366) with PBS. This raises concerns about its...
30 July 2025 8,184 2 View
I prepared a 1 mM stock solution of Decitabine in DMSO and added 1.32 μL to 4 mL of culture medium (final concentration: 330 nM) to treat Burkitt’s lymphoma cell lines. The cells were cultured...
28 July 2025 939 3 View
I plan to use HY-D0079 (Dihydroethidium) to detect ROS in frozen sections. Which fluorescence channel is recommended for detection?
28 July 2025 8,738 2 View
I’m currently attempting to dissolve ISX-9 for induction experiments but encountered precipitation issues. Here’s my protocol and the problem: Initial Dissolution: Dissolved 50 µg ISX-9 in 500 µL...
24 July 2025 8,753 1 View
Can you suggest a method to monitor cell viability in vitro using an immunofluorescence microscope from cell culture? I have PI and Texas Red, but I am not sure about the correct protocol. Thanks!
22 July 2025 7,197 4 View
I recently purchased the MCE compound Artemitin (Artemitin), item number: HY-N3017, which belongs to the flavonoids. I would like to ask how to dilute the product in cell experiments? At present,...
22 July 2025 963 2 View
I am planning to use Fura-2 AM to detect calcium levels in primary neurons, but the experimental methods in the references I searched for are somewhat different. In terms of reagent preparation,...
18 July 2025 429 1 View
I purchased the ROS probe H2DCFDA (DCFH-DA). Can I observe it under a fluorescence microscope after adding it to adherent cells? How long after staining can I perform the observation?
18 July 2025 7,538 2 View
While IV delivery would be more convenient and offer faster onset, I’m unsure whether GNF362’s physicochemical properties (e.g., solubility, stability) are suitable for this route. Additionally,...
16 July 2025 2,490 1 View
This compound is a NEDD4-1 inhibitor with demonstrated anticancer activity. Literature indicates it specifically suppresses the proliferation of human melanoma cells, which is highly relevant to...
16 July 2025 7,567 2 View
Despite carefully assembling the sandwich structure (8% SDS-PAGE gel to nitrocellulose membrane) and using a Teflon roller to remove bubbles, I consistently observe minor bubbles after overnight...
14 July 2025 3,907 2 View
I purchased HY-D1055 (MitoSOX Red) from MCE to detect mitochondrial ROS. However, the staining results showed strong fluorescence signals not only in mitochondria but also in the nucleus.
08 July 2025 8,790 2 View
How should 5-BrdU-labeled cells be processed for flow cytometry analysis?
08 July 2025 6,666 2 View
My siRNA-mediated gene knockdown isn’t working well—any troubleshooting suggestions? I’ve checked transfection efficiency, but the silencing effect remains weak.
03 July 2025 6,651 2 View
Seeking CCL3 inhibitors that effectively block the CCL3-CCR5/CCR1 axis in AD, preferably with evidence of crossing the BBB and modulating neuroinflammatory pathways (e.g., NF-κB/BACE1...
03 July 2025 3,713 1 View
My study uses an Aβ1-42-induced Alzheimer’s disease rat model, and I plan to administer both Aducanumab and Lecanemab as positive controls. However, I am unsure about the optimal dosing regimen...
01 July 2025 9,982 1 View
Does the 10 mg labeling on Nab-Paclitaxel (HY-P99974, MCE) indicate the powder weight or pure paclitaxel? Seeking advice from those who've used it for animal studies!
30 June 2025 163 3 View
I'm planning to use these peptides in a study on type 2 diabetes mellitus (T2DM). Does anyone know the key differences between [Pyr1]-Apelin-13 (HY-P1033) and Apelin-13 TFA (HY-P1944A)? I'm really...
26 June 2025 4,778 2 View
