Chloe Sun

16 Questions 1 Answers 0 Followers

Questions related from Chloe Sun

How water-soluble is Losartan?

What is the solubility of Losartan (HY-17512) in DPBS or water?

2 weeks ago 5,628 1 View

Do CD40L/CD154/TRAP protein, Canine (HEK293) and CD40 protein, Canine (HEK293, His) have the same effect on B cell activation?

I'd like to use a recombinant protein to activate B cells, but I'm unsure which one to choose. If anyone has relevant experimental experience to share, I'd be very grateful!

2 weeks ago 7,852 1 View

Can PEG8000 be used to dissolve drugs for animal experiments?

Etomoxir (HY-50202) is a carnitine palmitoyltransferase 1a (CPT-1a) inhibitor. Perhexiline maleate (HY-B1334A) is an orally active inhibitor of both CPT1 and CPT2. Can they be dissolved using PEG8000?

4 weeks ago 4,393 1 View

What are the possible causes of cellular abnormalities?

I am using Sorafenib on Huh7 and HepG2 cells. At 150 µM in 0.5% DMSO I observed something unusual in the drug-treated cells, not seen in the DMSO control. Could this be contamination or improper...

last month 4,608 2 View

Can WB/IP Lysis Buffer be used for protein extraction?

I plan to purify a protease product from mouse cell samples and subsequently characterize its activity. Is the MCE HY-K1000 kit suitable?

last month 383 4 View

Which form of enalapril—enalapril sodium, enalapril maleate, or enalaprilat dihydrate—is most suitable for oral administration?

I am currently conducting a research study and am interested in using enalapril as a positive control in my animal model (Wistar rats). Specifically, I am looking for a water-soluble form of...

last month 4,339 1 View

What is the dissolution method for GSKJ4 in animal experiment?

I’m using a diabetic nephropathy mouse model and planning to give the drug at 20 mg/kg via IP injection. Do you think normal saline (0.9% NaCl) would work for dissolving it, or should I consider...

last month 2,983 1 View

Which functional groups can AF 647 carboxylic acid label?

I am considering using AF 647 carboxylic acid (HY-D2160) to label CD19 protein, but I’m unsure whether it’s feasible. Thus, I’d like to confirm which functional groups AF 647 carboxylic acid can...

last month 7,708 1 View

Why is Roxadustat, a PHD inhibitor, not reducing PHD expression? Could there be a problem with my Western blot data?

In my experiment, PC12 cells were treated with 50 μM Roxadustat for 6 hours, followed by protein extraction and Western blot detection.

last month 1,280 1 View

Why was there no significant change in GPX4 protein levels in my RSL3-induced ferroptosis mouse model?

Observation: In my RSL3-induced ferroptosis mouse model, no significant reduction in GPX4 protein levels was observed under certain treatment conditions. Experimental Details: RSL3...

30 July 2025 8,468 1 View

Why does Clozapine N-oxide precipitate after dilution in PBS, and how can I prevent it?

Hi everyone, While performing a cell experiment, I noticed that precipitation occurs when I dilute the stock solution of Clozapine N-oxide (CNO, HY-17366) with PBS. This raises concerns about its...

30 July 2025 8,146 2 View

Anyone successfully used ISX-9 (Isoxazole 9) for stem cell differentiation?

I’m currently attempting to dissolve ISX-9 for induction experiments but encountered precipitation issues. Here’s my protocol and the problem: Initial Dissolution: Dissolved 50 µg ISX-9 in 500 µL...

24 July 2025 8,743 1 View

Could you provide a detailed protocol for performing immunofluorescence staining on cultured cells?

Can you suggest a method to monitor cell viability in vitro using an immunofluorescence microscope from cell culture? I have PI and Texas Red, but I am not sure about the correct protocol. Thanks!

22 July 2025 7,186 4 View

Can GNF362 be administered intravenously to induce the MCAO model?

While IV delivery would be more convenient and offer faster onset, I’m unsure whether GNF362’s physicochemical properties (e.g., solubility, stability) are suitable for this route. Additionally,...

16 July 2025 2,473 1 View

What strategies can be employed to improve the gene silencing efficiency of siRNA-based products?

My siRNA-mediated gene knockdown isn’t working well—any troubleshooting suggestions? I’ve checked transfection efficiency, but the silencing effect remains weak.

03 July 2025 6,642 2 View

For Alzheimer's research, which CCL3 inhibitors show the most promise?

Seeking CCL3 inhibitors that effectively block the CCL3-CCR5/CCR1 axis in AD, preferably with evidence of crossing the BBB and modulating neuroinflammatory pathways (e.g., NF-κB/BACE1...

03 July 2025 3,709 1 View