I want to use the H2DCFDA probe to detect changes in ROS levels in cells after viral infection. However, since the virus cannot be taken out of the high-level laboratory for flow cytometry, I would like to know whether the cells can be fixed with something like paraformaldehyde after incubation and then detected. Will this affect the results? Additionally, my virus carries a GFP fluorescent tag-could this factor influence the results?
1. In theory, after dyeing, the short-term fixation does not have much impact, and there is no specific time. Our customers usually perform double dyeing operations within 10 minutes, and the impact is not significant.
2. Labeling with GFP fluorescence will affect the experimental results, as it is also green fluorescence and H2DCFDA is also green, causing cross-over. It is not recommended to use them together.
You can consider using yellow fluorescent dye, HKPerox-1 (HY-130022)
The answer to this question is provided by MedChemExpress Technical Support.
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