I plan to use HY-D0079 (Dihydroethidium) to detect ROS in frozen sections. Which fluorescence channel is recommended for detection?
Chloe Sun, you have purchased our (MedChemExpress, MCE) Dihydroethidium (Cat.No.: HY-D0079) product.
The maximum emission wavelength of the Dihydroethidium dye itself is 420 nm, which can be observed through the DAPI channel.
After the dye is oxidized by oxides and binds to DNA or RNA, its maximum emission wavelength becomes 610 nm, and the PE channel can be used for detection.
The specific operation needs to refer to the parameter settings of the microscope or flow cytometer used.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
After reaction with superoxide the RFP Channel would be perfect.
https://doi.org/10.1002/adfm.201909839 --> Figure 15
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