This compound is a NEDD4-1 inhibitor with demonstrated anticancer activity. Literature indicates it specifically suppresses the proliferation of human melanoma cells, which is highly relevant to my current research. I plan to further investigate its effects by establishing an animal model to assess tumor suppression in mice. However, I have been unable to find relevant publications detailing experimental methodologies for reference.
Dear Chloe,
Do you have any demands for this inhibitor? You can refer to this product from TargetMol (https://www.targetmol.com/compound/1-benzyl-i3c) and technical support will be provided if you have product related questions.
Chloe Sun , yes, it's recommend to refere the PMID: 20570586 and PMID: 29028954.
These two articles studied the in vivo growth inhibitory efficacy of 1-Benzyl-I3C in a melanoma xenograft model and a melanoma xenograft model using athymic nude mice, respectively. The experimental design (partial) is as follows:
Melanoma Model establishment (PMID: 20570586):
Model establishment: G361 melanoma cells were injected into the flanks of nude mice to form palpable tumor xenografts.
- Treatment group: 1-Benzyl-I3C (20 mg/kg/day, subcutaneous injection);
- Control group: DMSO solvent (subcutaneous injection);
- Positive control (historical data): I3C (200 mg/kg/day, subcutaneous injection).
- Detection indicators: Tumor volume was measured weekly, tumor tissues were collected at the end of the experiment, and Wnt/β-catenin pathway-related proteins (such as LRP6, β-catenin, GSK-3β, etc.) were detected by immunofluorescence.
Breast cancer Model establishment (PMID: 29028954):
Model establishment: MCF-7 cells (estrogen receptor positive) were mixed with matrix gel and inoculated on the bilateral flanks of nude mice; MDA-MB-231 cells (estrogen receptor negative) were directly inoculated.
- Treatment group: 1-Benzyl-I3C (30 mg/kg/day, subcutaneous injection);
- Control group: DMSO solvent (subcutaneous injection);
- Positive control: I3C (300 mg/kg/day, subcutaneous injection).
- Detection indicators: Tumor volume was measured weekly, tumor morphology was recorded by taking photos at the end of the experiment, and the degree of vascularization was evaluated.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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