While I have reviewed several previous studies involving OGT2115, I have found considerable variation in the solvent compositions used to dissolve the compound. Therefore, I would like to inquire about the recommended solvent formulation—specifically the appropriate percentages of DMSO, PEG400, Tween-80, and saline—for administering OGT2115 via oral gavage or intraperitoneal injection in mice. Any guidance or established protocols would be greatly appreciated.
Generally speaking, for normal mice, the commonly used ratio is 10% DMSO+40% PEG300+5% Tween-80+45% Saline.
For weak mice such as those with immune deficiency or gene elimination, it is recommended to reduce the proportion of DMSO : 2% DMSO+40% PEG300+5% Tween-80+53% Saline.
Both clear or uniform suspensions can be used for intraperitoneal or oral gavageadministration.
However, for the OGT2115 product, we tested that this scheme is precipitated and is not recommended.
If you have a low dose (≤1.14 mg/mL) and a small number of dosings （≤ half a month）, you can consider Protocol 1 : 10% DMSO+ 90% Corn Oil.
If you have a high dose, you can consider the 0.5% CMC-Na/saline water ready-to-use scheme.
Both clear or uniform suspensions can be used for intraperitoneal or intragastric administration.
The answer to this question comes from MedChemExpress Technical Support.
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