I am using Sorafenib on Huh7 and HepG2 cells. At 150 µM in 0.5% DMSO I observed something unusual in the drug-treated cells, not seen in the DMSO control. Could this be contamination or improper dissolution?
Hello Chloe Sun
It is more likely to be cytotoxicity or precipitation from high concentrations of sorafenib or improper dissolution rather than contamination. Sorafenib may not dissolve completely in DMSO at such high concentrations, leading to precipitation and potential toxicity in culture. You may need to use a lower concentration of sorafenib to avoid overwhelming the cells with the drug or the DMSO solvent. Ensure your sorafenib stock solution is completely clear and free of any visible particles.
A 0.5% DMSO concentration is on the higher end and can be toxic to cells. Consider creating a new stock at a lower concentration that can then be diluted into your media with a lower final DMSO percentage. I feel you should carefully re-examine your DMSO control cells to ensure they are healthy and show no signs of stress or damage.
You should try several doses (0–20 μmol/L) of sorafenib. Please refer to the article attached below for more information.
Article Sorafenib inhibits proliferation and invasion of human hepat...
Best,
Sorafenib has poor water solubility, so precipitation at high concentrations is likely. Use ultrasonic heating at 37 °C to assist dissolution. Ensure both stock and diluted solutions are completely clear; filter and sterilize if needed before adding to cells.The answer to this question is provided by MedChemExpress Technical Support.
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