Type VII collagenase (HY-E70005J) is used to establish a rat model of intracerebral hemorrhage, with an administration concentration of 0.3 U/μL. How can the stock solution and working solution be prepared?
The COA document for this product provides the corresponding potency information, which can be referenced. Based on the product’s potency and the prepared mass concentration, the unit U/μL can be calculated. HBSS (Hank’s Balanced Salt Solution, containing Ca²⁺ and Mg²⁺) can be used to prepare the stock solution, which should be aliquoted and frozen for storage to avoid repeated freeze-thaw cycles. Subsequently, prior to experimental use, the stock solution can be diluted to the working concentration with PBS or physiological saline. The answer to this question is provided by MedChemExpress Technical Support.
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