Dear Researchers,

WB(IP) buffers are particularly used to extract protein from soluble animal cells and tissues.

These buffers have protein and phosphatase inhibitors activity, to prevent protein protein degradation and maintain protein modification in concern to downstream processing like SDS Phage and western blotting.

These resources discuss the importance of buffer choice depending on the characteristics of your protein of interest, and may be very useful for you.

https://pubmed.ncbi.nlm.nih.gov/30426406/

Article Protein Solubilization: Attend to the Choice of Lysis Buffer

MCE WB/IP Lysis Buffer is a lysis buffer used to lyse cell or tissue samples under non-denaturing conditions to prepare protein samples. This means it does not contain SDS and can be suitable.

However, it contains various inhibitors such as sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, EDTA, and leupeptin, which inhibit proteases and effectively prevent protein degradation and maintain the original protein-protein interactions. Because you want to characterize the activity of a protease, I would recommend a modified RIPA buffer that does not contain SDS. I would also be very cautious about any added protease inhibitors, because they may inhibit the protease of your interest.

Yes, it is feasible.

MCE WB/IP lysis buffer is a buffer used to lyse cells or tissue samples under non-denaturing conditions to prepare protein samples. The lysed cell or tissue samples can be used for PAGE, Western Blot, immunoprecipitation (IP), co-immunoprecipitation (co-IP), and ELISA.

The main components of the WB/IP lysis buffer are sodium pyrophosphate, β-glycerophosphate, sodium orthovanadate, EDTA, and leupeptin, which can inhibit protein degradation and dephosphorylation.

The answer to this question comes from MedChemExpress Technical Support. [email protected]