Is flow cytometric analysis suitable for 5-BrdU-stained cells?

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Lucian Miles

Chloe Sun ,

Yes. 5-BrdU (HY-15910, MCE) is incorporated into the synthesized DNA during cell division, and its signal is localized in the nucleus. If flow cytometry is used, the membrane needs to be broken and pores need to be punched when incubating with antibodies.

The answer to this question comes from MCE Technical Support.

[email protected]

Sidney T. Sithole

Chloe Sun Yes, 5-BrdU labeled cells can be analyzed by Flow Cytometry. I've found the ThermoFisher protocol useful, however, you might need to optimize it for best results depending on your cell line/cell type. Also, take note to include proper controls: unstained, no-BrdU, and isotype controls as needed.

BestProtocols: BrdU Staining Protocol for Flow Cytometry | Thermo Fisher Scientific - US.