56 Questions 1 Answers 0 Followers
Questions related from Yan Yan Zhao
3-indoleacetic acid (HY-18569) 1. Is ultrasonic dissolution required for both water dissolution and DMSO dissolution as indicated in the instruction manual? Since our experimental conditions do...
5 days ago 8,361 1 View
Hello everyone, I would like to ask how to optimize the Western blot experiment for TMS1/ACS Antibody (HY-P80548) and Caspase-1 Antibody (HY-P81232), as the bands are very faint and there are...
6 days ago 7,526 2 View
Currently, we plan to use AS1842856 administered via intraperitoneal injection to intervene in the FOXO1 pathway in a rat model of coronary heart disease. In SD rat experiments, can AS1842856...
2 weeks ago 2,782 0 View
Hello everyone, may I ask what the dosage of SIRT2/6-IN-1 (HY-123194, MCE) is for intraperitoneal injection in rats? We have read many references but were unable to find the exact dosage for rats.
2 weeks ago 1,541 1 View
I would like to use Lactic acid, Biotin labeled (HY-B2227F, MCE) for immunoprecipitation (IP). The presence of DMSO might interfere with the binding to the magnetic beads, so I would like to know...
2 weeks ago 6,680 1 View
Hello everyone, may I ask how to dissolve Methylprednisolone Sodium Succinate (HY-B1060, MCE)?
2 weeks ago 4,935 4 View
I want to conduct an experiment using ketoconazole (HY-B0105, MCE) to inhibit CYP3A4. I treated HepG2 cells with 0-10 μM ketoconazole for 24 hours and then measured the mRNA level of CYP3A4, but...
2 weeks ago 2,492 1 View
Hoechst33342 (HY-15559, MCE) was used for tail vein injection in mice. The concentration reported in the literature was 4.8 mg/mL dissolved in normal saline, with 50 μL administered per mouse....
3 weeks ago 5,594 1 View
Hello everyone, may I ask how should Dihydrotanshinone I (HY-N0360, MCE) be dissolved for use in a subcutaneous tumor formation experiment? The compound is administered after tumor formation at a...
3 weeks ago 8,826 1 View
What is the solubility of Streptonigrin (HY-124586, MCE) in water? Can Streptonigrin be dissolved directly by adding 1mL of DMSO without freeze-drying?
3 weeks ago 4,708 3 View
Chloroquine (HY-17589A, MCE) purchased in 2022 has been stored at -20°C. Several experiments were conducted in the field of targeted protein degradation, and it was found that chloroquine could...
3 weeks ago 9,946 2 View
How should GW4869 (HY-19363, MCE) be dissolved and administered in animal experiments?
3 weeks ago 2,860 1 View
After infecting Hepa1-6 cells with an empty virus vector and a specific library, the cells were resuspended in BODIPY 493/503 (HY-W090090, MCE) fluorescent dye at a working concentration of 2 µM...
4 weeks ago 991 1 View
I am planning to use siRNA for SLC35F3 I am wondering if siRNA/miRNA Transfection Reagent (HY-K2017) can be used in Amaxa 4D preparations for nucleofection.
4 weeks ago 7,501 2 View
Hello everyone, may I ask what the activity assay method is for NRG1-beta 1 Protein, Human (HEK293) (HY-P73328, MCE), using regular MCF-7 cells without genetic engineering?
4 weeks ago 7,862 1 View
Regarding CQ-Lyso (HY-D2119, MCE), I have a few questions: What is the suitable pH for the B-R buffer used to prepare the CQ-Lyso working solution? Is there detailed instructions on how to...
4 weeks ago 3,837 1 View
When performing CCK-8 assays on the HCC95 cell line (concentration gradient 0–20 mM), no obvious dose-response trend was observed. Are there any literature reports supporting the use of...
5 weeks ago 2,323 1 View
Are there inhibitors for the P53 apoptosis pathway, including the intrinsic mitochondrial apoptosis pathway and the extrinsic death receptor pathway?
last month 4,595 3 View
Can Fexagratinib (HY-13330, MCE) be administered to animals if it is a suspension?
last month 4,663 1 View
D-luciferin suitable for in vivo luminescence experiments in mice. (Luminescence from a mouse model of cancer caused by cancer cells transfected with the firefly luciferase gene → evaluation by...
last month 470 3 View
I'm doing adult fruit fly feeding assay. I test with 1 mM DMSO solution, flies died in two days because of DMSO. Does anyone know of other solvents (such as water, ethanol, or acetone) that can...
last month 5,427 1 View
I’m having issues with Vemurafenib(HY-12057, MCE) as it seems to be forming crystals in cell culture - the crystals are those dark structures in the culture, the cells underneath have mostly died....
last month 5,700 1 View
The LQZ-7F product (Catalog Number: HY-122678) is planned for use in animal experiments. Specifically, it will be administered via intraperitoneal injection after tumor formation in nude mice,...
last month 2,768 2 View
For the compound BzATP triethylammonium salt (HY-136254), when performing microinjections into specific brain regions of rats, what is the recommended dosage, frequency, and duration of...
last month 5,130 1 View
Hello everyone, why did the color of Serum/Protein-Free Cell Freezing Medium (HY-K1012, MCE) change during use?
last month 456 1 View
Hello everyone, how should oleic acid (HY-N1446, MCE) and palmitoleic acid (HY-W011873, MCE) be dissolved for in vivo studies in mice? The study will use 8-week-old female C57 mice (average weight...
last month 168 1 View
Hello everyone, I would like to ask: For 4-PBA (HY-A0281, MCE), with a dosing concentration of 100 mg/kg and a dosing volume of 200 μL, how much should I prepare for 7 days of administration? The...
last month 7,369 1 View
I have an in vivo experiment in mice, in which I want to inject Olaparib ( HY-10162, MCE) intra-peritoneal. To prepare Olaparib, I first need to suspend it in DMSO, then add 20% SBE-β-CD...
last month 6,757 1 View
Can AZD8797 (HY-13848, MCE) be formulated with 10% DMSO + 90% saline for in vivo mouse models?
last month 4,839 2 View
Are tacrolimus (HY-13756, MCE) and plerixafor (HY-10046, MCE) used together in combination? Are they injected into the same mouse, or administered separately into two different mice (i.e., as part...
last month 3,702 1 View
We plan to dissolve the enzyme (HY-P2863,MCE) in ultrapure water to create a concentrated stock solution, as recommended. We will then prepare small aliquots of this stock, freeze them at -20°C,...
last month 383 1 View
Hello everyone, I’ve recently been using Ruxolitinib (HY-50856, MCE) for cell experiments. Is this compound lipid-soluble or water-soluble?
last month 8,812 1 View
Hi all, can the dye Biotin TSA (200×)(HY-D1839, MCE) be diluted directly with PBS?
last month 8,009 2 View
What are the differences between Bradford (HY-K2001, MCE) protein quantification and BCA quantification?
last month 2,723 3 View
Hi all, What is the inhibitory mechanism of ustekinumab (HY-P9909, MCE)? Is it a dual-target inhibitor?
last month 2,714 2 View
Hello everyone, could you explain the staining mechanism of TO-PRO-3 iodide (HY-117070, MCE)?
01 August 2025 4,935 1 View
Hello everyone, Recently, I detected the expression of p-p65 ( HY-P80470, MCE) and p-IKBα ( HY-P80466, MCE) in HSF cells by Western blot , but the results seem problematic—no bands appeared, and...
31 July 2025 3,080 1 View
I couldn't find relevant information in the literature, but the product manual states that it can be used for in vivo injection. I want to administer a certain dose of 12(S)-HHT (HY-113330, MCE)...
29 July 2025 6,662 1 View
Hi all, can PolyFast (HY-K1014, MCE) be used to transfect suspension cells?
28 July 2025 4,752 1 View
I treated prostate cancer cell lines LNCaP and 22Rv1 with 10 μM and 20 μM Enzalutamide (HY-70002, MCE), but no significant cytotoxic effects were observed.
25 July 2025 422 1 View
After dissolving CAY10566 (HY-15823, MCE) in DMSO, insoluble particles (precipitates) were observed. Despite trying other solubilization methods, the issue persisted. The attached image shows the...
24 July 2025 5,795 1 View
In my experiment, I needed to prepare a cholesterol (HY-N0322, MCE) solution. I attempted to dissolve solid cholesterol in ethanol (20 mg/mL), but it took an extremely long time to dissolve and...
23 July 2025 8,150 2 View
After treating the U2OS cell line with three different concentration gradients of the TLK2 inhibitor UNC-CA2-103 (HY-162426, MCE) for 48 hours, I extracted proteins and performed Western blotting...
22 July 2025 7,434 2 View
I have been trying to grow the Medulloblastoma cell line D341 for weeks now but they are extremely slow growing! I used the exact media and supplementation suggested by the ATCC. These cells grow...
21 July 2025 717 1 View
I have been working with cell lines that I have never worked with before, HUVEC and HT29 , the problem started with HT29 then I started seeing in HUVEC also, I would be maintaining the cells...
18 July 2025 6,601 2 View
Could you recommend the volume of PKH26 (HY-D1451,MCE) working solution required to completely stain 100 μg/mL of exosomes, while ensuring clearly observable staining in vivo imaging?
17 July 2025 6,275 1 View
I am planning a mouse experiment to investigate the extended biological functions of Semaglutide (HY-114118, MCE). Could experienced researchers share key considerations for animal dosing in such...
16 July 2025 9,320 1 View
Recently, I have been doing experiments on plant stress response, involving salicylic acid (SA), jasmonic acid (JA), and methyl jasmonate (MeJA). I want to use inhibitors to specifically block...
15 July 2025 3,358 1 View
Do you have any recommended Lamin B1 antibodies for obtaining high-quality fluorescence microscopy images?
14 July 2025 255 1 View
Hi all, On Day 3 of a 6-week Caco-2 cell cancer study, after changing the medium in T75 flasks and centrifuging the samples, half of the healthy cells died and failed to reattach. Why did this happen?
11 July 2025 3,454 2 View
Is Formamide (HY-Y0842, MCE) Toxic? What precautions should be taken during handling?
08 July 2025 5,884 3 View
Hi all, Will it affect antibody staining if paraffin tissue is fixed for 3-4 days?
04 July 2025 4,573 2 View
Hi all, what are the differences between these cell lysis agents: Digitonin (HY-N4000), NP40 and Triton x-100?
03 July 2025 4,554 1 View
Hello everyone, what is the difference between Sulfo-Cyanine5 (Cy5) (HY-D0821) and Cy5 NHS Ester (HY-D0819), and which one is more suitable for live cell tracing?
02 July 2025 1,846 3 View
Could you provide recommendations on whether HY-D0079 Dihydroethidium (Hydroethidine; PD-MY 003) is suitable for tissue staining applications?
27 June 2025 1,783 1 View
I would like to know if Protein A/G Magnetic Beads (HY-K0202) can be used to purify hybridomas or ascites. Could you provide the detailed purification protocol? Thank you very much.
26 June 2025 3,837 1 View
