Hello everyone, may I ask how should Dihydrotanshinone I (HY-N0360, MCE) be dissolved for use in a subcutaneous tumor formation experiment? The compound is administered after tumor formation at a dosage of 10 mg/kg, and the nude mice weigh approximately 20-25 g.
A formulation of 10% NMP + 90% PEG300 provides a clear solution with a solubility of 2.5 mg/mL. For intraperitoneal administration in mice, an alternative suspension formulation can be considered, consisting of 5% NMP (HY-Y1275) + 5% Solutol HS-15 (HY-Y1893) + 30% PEG400 + 60% saline, which supports a solubility of 1.25 mg/mL. It is advisable to first prepare a stock solution in NMP before dilution to the final working concentration.
The answer to this question comes from MCE Technical Support. [email protected]