3-indoleacetic acid (HY-18569)
1. Is ultrasonic dissolution required for both water dissolution and DMSO dissolution as indicated in the instruction manual? Since our experimental conditions do not permit, is there any other way to assist in dissolution? If DMSO is used for dissolution, ultrasonic waves can be avoided.
2. What is the minimum amount of DMSO reagent that causes damage to cells?
3. If 5g of 3-indoleacetic acid is dissolved in DMSO (I want to dissolve this into the highest concentration of the original solution that can be dissolved, and then the final concentration is obtained by diluting the original solution) for the CCK-8 experiment, and the final volume of each well in the 96-well plate is 100 μL, then the final concentrations I want are 1 mM,5 mM, 10 mM. How to dissolve it.
3-Indoleacetic acid (3-IAA) is best dissolved in DMSO with gentle heating or vortexing to aid solubility without ultrasound; keep DMSO concentration below 0.5-1% in cell assays to avoid toxicity, and to prepare stock for CCK-8 assays at 1, 5, 10 mM final concentrations in 100 μL wells, dissolve 5 g in ~100 mL DMSO for a ~285 mM stock, then dilute accordingly.