Hi all, can the dye Biotin TSA (200×)(HY-D1839, MCE) be diluted directly with PBS?
Direct dilution with PBS is not recommended. The signal amplification of Biotin TSA relies on the HRP-catalyzed activation of tyramide, which requires a low concentration of hydrogen peroxide (0.001%–0.03%) for optimal reaction. PBS lacks essential components (e.g., H₂O₂) and may reduce reaction efficiency or cause failure. It is advised to use the recommended dilution buffer (e.g., HY-D1840), which is formulated to maintain reagent stability and support the reaction conditions.
The answer to this question comes from MCE Technical Support.
Yes, Biotin TSA (200×) dye can be diluted directly with PBS, but this is generally not recommended. The TSA (Tyramide Signal Amplification) reaction requires optimal conditions, including hydrogen peroxide (H₂O₂) and a suitable buffer (often borate buffer, pH ~8.5), which PBS does not provide. The manufacturer’s recommended dilution is usually in a proprietary amplification diluent to ensure proper tyramide deposition, signal strength, and minimal background. If PBS must be used, adding 0.0015–0.003% H₂O₂ may help, but results may be inconsistent with higher background or reduced sensitivity. For reliable performance, always follow the kit’s protocol or use a compatible buffer system.
- Badges
- Science topic
- Similar topics
- Engineering
- Materials Engineering
- Powders