Hoechst33342 (HY-15559, MCE) was used for tail vein injection in mice. The concentration reported in the literature was 4.8 mg/mL dissolved in normal saline, with 50 μL administered per mouse. However, during actual operation, I found that neither the 4.8 mg/mL concentration nor a dilution by half could achieve complete dissolution, even when heated to 50 degrees Celsius. According to my research, the solubility is stated to be 10-20 mg/mL, so I am unsure why it fails to dissolve.
HY-15559 is practically insoluble in water based on our testing. To achieve a concentration of 4.8 mg/mL for intravenous administration via the tail vein, current dissolution methods for HY-15559 are insufficient. Our DMSO-based formulation can only reach a working concentration of approximately 0.5 mg/mL.
The reference where it was dissolved in physiological saline likely refer to its salt form. We also offer an equivalent salt form product, HY-15559A, which has relatively better water solubility.
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