I have an in vivo experiment in mice, in which I want to inject Olaparib ( HY-10162, MCE) intra-peritoneal. To prepare Olaparib, I first need to suspend it in DMSO, then add 20% SBE-β-CD (HY-17031, MCE) in saline. The Olaparib in DMSO I know I have to filter.
1. Do I need to filter the 20% SBE-β-CD in saline solution before use? if yes, which filter is suitable?
2. Can I aliquot and freeze the final mix (ready for injection) and keep it for 1 month in -20℃, so that I can thaw and use one aliquot every day for the injection?
If not, can I freeze the aliquots of Olaparib-DMSO and the 20% SBE-β-CD in saline solution separately and mix at the day of injection?
1. Animal experiments generally do not require filtration because the dissolved solution is viscous and not suitable for filtration. DMSO itself has bactericidal properties.
2. 20% SBE-β-CD in saline solution is also not recommended for filtration. If you are really worried, you can have a short period of ultraviolet irradiation.
3. It is not recommended to package and freeze the final mix (ready for injection) as it is difficult to resuspend and does not guarantee activity.
4. It is suggested that the Olaparib-DMSO can be divided into -20°C for one year and -80 °C for two years to avoid repeated freezing and thawing.
It is recommended to store 20% SBE-β-CD saline solution at 4°C for a maximum of one week. After more than a week, it is recommended to reconfigure.
The answer to this question comes from MedChemExpress Technical Support.
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