Could you recommend the volume of PKH26 (HY-D1451,MCE) working solution required to completely stain 100 μg/mL of exosomes, while ensuring clearly observable staining in vivo imaging?
PKH26 utilizes a dyeing mechanism that binds to lipid regions and is commonly used for cell labeling in cell proliferation assays. However, exosomes differ from cells in terms of size and characteristics, with their dimensions being much smaller than those of cells. When drawing an analogy to cell labeling, a significantly smaller volume of PKH26 working solution may be required for a given amount of exosomes compared to cell labeling.
Considering an exosome concentration of 100 μg/mL and a sample volume of 1 mL, the estimated volume of the PKH26 working solution, based on the quantity used for cell staining, may be in the range of several tens of microliters. The precise amount, however, should be optimized through pilot experiments.
Here is a recommended publication that involves the injection of PKH26-labeled BMSCs into rats for in vivo imaging:Article Comparative Study of the Rat-Labeled PKH26-MSCs and Its Deri...
The answer to this question comes from MedChemExpress (MCE) Technical [email protected]
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