I treated prostate cancer cell lines LNCaP and 22Rv1 with 10 μM and 20 μM Enzalutamide (HY-70002, MCE), but no significant cytotoxic effects were observed.
I found that the concentration generally used in the literature is also around 20 μM, and the treatment is 48 and 72 hours. It may be that your cells grow fast, resulting in a lack of obvious inhibitory effect when adding drugs. It is recommended that the number of cells should not be too large when adding drugs at the beginning, and it is more appropriate to adjust it to 50-60% of the current level. It may also be that the difference between different cell states is large, so some literature will use 40 μM treatment.
（HY-70002）https://pmc.ncbi.nlm.nih.gov/articles/PMC9344336/ (Enzalutamide's IC50 for LNCAP is 14.3 μM)
https://www.nature.com/articles/s41598-020-80519-3 (Enzalutamide began to significantly inhibit 22RV1 at a concentration of 40 μM, and the treatment time was 48 h and 72h)
The answer to this question comes from MedChemExpress (MCE) Technical Support.