Yan Yan Zhao , NP-40 and Triton X-100 are both commonly used cell lysis reagents, and their main function is to completely dissolve the cell membrane to release proteins and other components in the cell. Digitonin is a mild selective cell lysis reagent, which is often used to separate the cytoplasm from the nucleus.

It should also be noted that Triton X-100 can not only dissolve the cell membrane, but also destroy the inner membrane structure such as the nuclear membrane to achieve more thorough cell lysis.

In different application scenarios, Digitonin is often used to separate cytoplasmic and nuclear components, and is suitable for experiments that need to retain the integrity of the nuclear structure (such as step-by-step analysis of nuclear protein extraction and cytoplasmic protein extraction). NP-40 is suitable for conventional cell lysis to release cytoplasmic components, and is often used to extract soluble proteins in experiments such as immunoprecipitation and Western blot, especially for scenarios where the nuclear structure does not need to be completely destroyed. Triton X-100: Because the lysis is more thorough, all soluble proteins in the cell, including nuclear components, can be released, and it is suitable for experiments that require comprehensive extraction of intracellular components (such as whole cell protein extraction).

The answer to this question comes from MedChemExpress (MCE) Technical Support.

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