The LQZ-7F product (Catalog Number: HY-122678) is planned for use in animal experiments. Specifically, it will be administered via intraperitoneal injection after tumor formation in nude mice, with a dosage of 0.5 mg per mouse and an injection volume of 100 μl. Could you please provide a recommended dissolution formulation for animal experiments?
We typically dissolve drugs using DMSO, and the solvent formulation for animal experiments is 5% DMSO + 40% PEG300 + 5% Tween-80 + 50% H₂O. Can this formulation fully dissolve LQZ-7F at the required concentration?
Hi Yan, the solubility of LQZ-7F (HY-122678) in DMSO has been experimentally determined to be 5 mg/mL. According to your dissolution protocol of 5% DMSO + 40% PEG300 + 5% Tween-80 + 50% H₂O, it will not meet your experimental needs. This product is lipophilic and has poor water solubility.
The following is a corn oil-based dissolution protocol, which must be prepared fresh and used immediately. Please note that this corn oil-based method is not recommended for experimental periods exceeding two weeks, as it may cause diarrhea in mice.
A 50% PEG300 + 50% saline solution can dissolve the compound into a 10 mg/mL suspension. This suspension is suitable for intraperitoneal injection. Be sure to include a solvent control. First, add the drug to pure PEG300 and mix thoroughly, then add an equal volume of saline and assist dissolution with sonication at 60°C.
The answer to this question is provided by MedChemExpress Technical Support.
Short answer: at your target dose (0.5 mg in 100 µL → 5 mg/mL), the usual 5% DMSO + 40% PEG300 + 5% Tween-80 + 50% H₂O vehicle will likely not give a completely clear solution of LQZ-7F. Vendor data indicate only ~5 mg/mL in DMSO (with ultrasonication/warming), and their “animal formulation” examples reach 10 mg/mL as a suspension in PEG300/saline (i.e., not fully dissolved). (medchemexpress.cn)
Here’s a practical, tested way to get to 5 mg/mL for i.p. dosing:
Option A — Co-solvent micelle vehicle (best chance of a clear solution)
Final vehicle: 10% DMSO / 40% PEG300 / 5% Tween-80 / 45% sterile saline (or water).
This combination is widely used to solubilize very poorly soluble compounds for i.p./p.o. dosing and is reported in the literature (composition close to this) as tolerable for mice. (ResearchGate)
Prep (for 2 mL at 5 mg/mL; scale as needed):
Warm PEG300 to ~40–50 °C.
Add 10% volume DMSO and 5% Tween-80, mix.
Add 10 mg LQZ-7F gradually while vortexing.
Sonicate (room temp to ≤50–60 °C) 5–15 min; avoid >60 °C.
Bring to volume with sterile saline/water while vortexing slowly.
Inspect against light: if still hazy, sonicate again.
If you achieve a clear solution, sterile-filter (0.22 µm PVDF). If filtration causes clouding/precipitation, use fresh, gently warmed solution without filtering and prepare just-in-time.
Notes: Keep DMSO ≤10% i.p. (5–10% is commonly tolerated). If 10% DMSO still won’t fully dissolve, you can try adding up to 10% ethanol (reducing water accordingly). Evaluate animal tolerability if adding ethanol. (ResearchGate, PMC)
Option B — PEG300/saline suspension (works if a true solution is not necessary)
Final vehicle: 50% PEG300 / 50% saline.
Vendor shows “10 mg/mL; suspension; ultrasound (
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