Hello everyone, how should oleic acid (HY-N1446, MCE) and palmitoleic acid (HY-W011873, MCE) be dissolved for in vivo studies in mice?
The study will use 8-week-old female C57 mice (average weight ~20g) with intraperitoneal (IP) injection at a volume of 200–300 µL/mouse. The mice are not immunodeficient or genetically modified, but surgical modeling is required before dosing.
Hi Yan, according to the information you provided:
Palmitoleic acid HY-W011873 The administration concentration is 6 mg/mL. Ultrasonically dissolve in 0.5% CMC-Na/saline solution to obtain a 20 mg/mL suspension, which can be used for intraperitoneal injection. Prepare fresh before use.
Oleic acid HY-N1446
Both of the above methods can meet your requirement of 6 mg/mL for intraperitoneal injection, and you may choose either option.
The answer to this question comes from MedChemExpress Technical Support.