This product is poorly water-soluble and is easily precipitated when directly added to saline after preparation with DMSO. DMSO itself is toxic to animals. For larger-volume administration methods (100-200 μL), such as intraperitoneal injection, it is generally recommended that the final concentration in healthy mice be controlled below 10%.

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AZD8797 can typically be prepared in a 10% DMSO + 90% saline solution for experimental use, but several critical factors must be considered to ensure optimal solubility, stability, and biological compatibility. As a CX3CR1 antagonist, AZD8797 demonstrates good solubility in pure DMSO (often 10-50 mM), and dilution to 10% DMSO in saline is generally suitable for in vitro applications at concentrations up to approximately 1 mg/mL. However, for in vivo administration, the DMSO concentration should ideally be reduced to 5% or less to minimize potential solvent-related toxicity, such as hemolysis or tissue irritation. When preparing the solution, it is essential to first dissolve the compound in 100% DMSO to create a concentrated stock solution before diluting with sterile saline to the desired final concentration, followed by gentle mixing and visual inspection for precipitation. The solution should be sterile-filtered (0.22 µm) if intended for cell culture or injection purposes. For cellular studies, the 10% DMSO concentration may require validation through cytotoxicity assays (e.g., MTT), as some cell lines exhibit reduced viability at this DMSO level. If solubility issues arise, alternative formulations incorporating co-solvents like PEG-300 (5-10%) or solubilizing agents such as cyclodextrins (10-20%) may be considered. Importantly, appropriate vehicle controls containing equivalent DMSO concentrations should always be included in experiments to account for potential solvent effects. The stability of AZD8797 in this solution should be monitored over time, particularly for long-term storage, where freezing at -20°C in aliquots is recommended to prevent repeated freeze-thaw cycles. For in vivo studies requiring higher doses, further dilution in saline to achieve ≤5% DMSO is strongly advised to ensure animal welfare while maintaining compound solubility.