I’m having issues with Vemurafenib(HY-12057, MCE) as it seems to be forming crystals in cell culture - the crystals are those dark structures in the culture, the cells underneath have mostly died. When looking through the microscope they appear brownish in colour. I reconstituted 2 mg in to 204 μL of DMSO to make a 20 mM stock concentration, which I then used at a final concentration of 20 μM (1:1000) in cell culture. Since I got these crystals in the first experiment I did last week, I made a new batch using the same amounts and volumes and used a bit of heat to help with dissolving (stock solution looked completely clear), but I got these crystals again using the new batch. Could there be an issue with this compound or is there potentially something I’m doing wrong?
This product is poorly water-soluble. In the case of the customer, precipitation is likely due to dilution in the culture medium.
It is recommended that after diluting the stock solution with culture medium to a working solution, examine the solution under a microscope to ensure clarity and to see if any crystals have formed. If precipitation is present, ultrasonic heating can be used to assist dissolution at 37°C. Once complete dissolution is achieved, replace the solution with the cells.
The answer to this question is provided by MedChemExpress Technical Support.
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