We plan to dissolve the enzyme (HY-P2863,MCE) in ultrapure water to create a concentrated stock solution, as recommended. We will then prepare small aliquots of this stock, freeze them at -20°C, and thaw only one aliquot per experiment to avoid repeated freeze-thaw cycles. What would be the estimate enzyme activity after one freeze and thaw cycle?
Qiu Weiwei
For HY-P2863, one cycle freeze and thaw will not affect the enzyme activity massively. The enzyme activities are 855 U/mg solid or 1025 U/mg solid, which are different according to the batches. The 5 KU package therefore equals to 5.848 mg or 4.878 mg solid. It's best to avoid repeated freeze-thaw cycles.
The answer to this question comes from MedChemExpress Technical Support. [email protected]
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