Hello everyone, I would like to ask: For 4-PBA (HY-A0281, MCE), with a dosing concentration of 100 mg/kg and a dosing volume of 200 μL, how much should I prepare for 7 days of administration? The mice weigh 20 ± 2 g, and the only solvents I have are DMSO and Tween 80.
Would you also be able to help calculate the required amount for one day's dose?
Hongya Cheng
Dissolve in DMSO to 500 mg/mL, then dilute to 10 mg/mL using a 2% DMSO + 40% PEG300 + 5% Tween-80 + 53% saline solution to obtain a clear solution. Take 5 mg of the compound and add 10 μL of DMSO to observe if it dissolves. Use sonication to aid dissolution. Sequentially add 200 μL of PEG 300 and mix well. Add 25 μL of Tween 80 and mix well. Finally, add 265 μL of saline and mix thoroughly. Dissolve to 20 mg/mL using a 15% Cremophor EL + 85% saline solution, resulting in a clear solution. Dissolve to 20 mg/mL using a 20% HP-β-CD in saline solution with sonication to achieve a clear solution. First, prepare a 20% HP-β-CD in saline solution using physiological saline. Then, use this solvent to dissolve the compound.
The concentration used in animal experiments is relatively high, so a cosolvent is required to assist in dissolution. Below are the tested feasible solutions. You can choose based on your specific situation.
The answer to this question comes from MedChemExpress Technical Support.
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