Chloroquine (HY-17589A, MCE) purchased in 2022 has been stored at -20°C. Several experiments were conducted in the field of targeted protein degradation, and it was found that chloroquine could not effectively inhibit the lysosomal pathway at concentrations of 10 μM and 20 μM. The Jurkat cell line was used, and the treatment method involved co-culturing the drug with chloroquine for 24 hours. Could this be due to drug degradation?
Hi YanYan, did you see my question about this issue? Thank you very much for sharing your experience. https://www.researchgate.net/post/Why_cant_I_reproduce_the_same_results_for_UNC-CA2-103_as_reported_in_the_literature
The compound has indeed been stored for quite a long time, so drug degradation cannot be ruled out.
You can also try increasing the concentration to compare and see if there is any difference. According to the literature, cell experiments have used concentrations as high as 50 to 120 μM.
If there is still no difference, I recommend purchasing a new compound for comparison and testing.
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