I am planning to use siRNA for SLC35F3 I am wondering if siRNA/miRNA Transfection Reagent (HY-K2017) can be used in Amaxa 4D preparations for nucleofection.
1. The Amaxa 4D Nucleofector nuclear transfection mechanism: Using a proprietary voltage pulse program, nucleic acids (such as DNA, siRNA, and mRNA) are directly delivered into the cell nucleus through the cell and nuclear membranes. This is accomplished by optimizing the electroporation pulse program (program code) and the accompanying dedicated buffer (Nucleofector Solution). No separate siRNA reagents are required. 2. The HY-K2017 uses a chemical transfection method and does not require the use of associated transfection equipment.
The answer to this question is provided by MedChemExpress Technical Support. [email protected]
Nucleofection is indeed efficient yet very toxic, and there is no need to add a transfection molecule to this physical system of genetic modification.
As a powerful alternative for siRNA/miRNA transfection, I would like to present you our Lullaby transfection reagent, dedicated togene silencing. It is a lipid-based reagent highly efficient that presents a very low index of toxicity.
A wide range of short nucleic acid sequences can be used without any problem and co-transfections as well as sequential transfection are also possible.
■ Moreover, among the hundreths of publications metnioning its use (http://www.ozbiosciences.com/module/citationfinder/default), Lullaby transfection reagent was selected by the Cancer Research UK Beatson Institute for siRNA screening approach and as stated by Emma J. Shanks, "... By far, our preferred reagent is Lullaby from OZ Biosciences. We have used this reagent in over 20 cell lines and have found it essential in enabling siRNA screening in hard-to-transfect cell lines such as those derived from GEMM pancreatic tumors and AML suspension cell lines, with minimal toxicity"1.
This is why I am confident that Lullaby would be the reagent of choice for your application.
If you need any information or if you want to try Lullaby, please do not hesitate to contact me via ResearchGate or directly at: [email protected]
best regards,
Cedric
PS: it is important to note that we also propose an alternative to electroporation using a physical method: the Magnetofection technology (very briefly: it uses a magnetic field to attract and concentrate magnetic nanoparticles complexed to nucleic acids or viral vectors - very efficient indeed!)