Could you provide recommendations on whether HY-D0079 Dihydroethidium (Hydroethidine; PD-MY 003) is suitable for tissue staining applications?
From the perspective of experimental technology, it is not recommended to use live cell dyes to stain tissue samples.
Although there are literature reports that such dyes can be used to stain tissue sections, two major technical problems are prone to occur in actual operation:
first, it will cause a significant increase in the staining background;
second, there is a high risk of false positive results.
This conclusion is based on the interaction characteristics of live cell dyes with tissue samples, that is, the dyes may undergo non-specific binding in the tissue environment, thereby affecting the accuracy and reliability of the staining results.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
- Badges
- Science topic
- Similar topics
- Microbiology
- Assays