Hi all, can PolyFast (HY-K1014, MCE) be used to transfect suspension cells?
Guidelines for Transfection in Suspension Cell Culture
Following transfection, it is recommended to reduce the agitation speed of the shaking incubator to facilitate sufficient contact between the PolyFast-DNA complexes and the cells, thereby enhancing uptake efficiency. This adjustment also helps minimize cell sedimentation, aggregation, or hypoxia during the critical transfection phase.
After 6 hours post-transfection, the agitation speed may be gradually restored to the routine setting to ensure optimal cell growth conditions while maintaining transfection efficacy.
This protocol aims to balance transfection efficiency with cell viability and is recommended for reproducible experimental outcomes. For further optimization, adjustments may be tailored to specific cell line requirements.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
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