Fan Janice

51 Questions 14 Answers 0 Followers

Questions related from Fan Janice

What are the effects of ML385 on HO-1, NF-κB, NQO1, and TNF-α expression across different cell types?

I am designing an experiment with the following groups: control, oxidized fish oil, oxidized fish oil + herbal medicine, and oxidized fish oil + herbal medicine + ML385 (HY-100523). The oxidized...

2 weeks ago 1,403 2 View

What are the optimal BrdU injection dose, frequency, and timing for IF staining in the mouse hippocampal DG region?

Hi all, regarding the 5-BrdU (HY-15910, MedChemExpress) product: has anyone optimized BrdU labeling in the mouse hippocampal DG? Specifically, what injection dose, frequency, and timing before...

3 weeks ago 3,097 1 View

What is the recommended solvent formulation for OGT2115 in mice (oral gavage or IP)?

Hi everyone, I’ve reviewed several studies on OGT2115 and noticed considerable variation in the solvent compositions used. Could anyone share guidance on the recommended formulation—specifically...

3 weeks ago 6,018 1 View

How should AZD0095 be dissolved?

Hi all, I need to prepare a gavage solution at 10 mg/mL (dose: 100 mg/kg, 200 μL for a 20 g mouse). Could you recommend the best solvent system or dissolution method? Thank you so much in advance!

3 weeks ago 1,086 1 View

How to avoid DMSO–corn oil phase separation when preparing MSAB for i.p. injection, and will PEG300/Tween80 solve it?

Hi everyone, I am doing an experiment involving MSAB (HY-120697) and according to the datasheet, MSAB dissolves well in DMSO at 10 mg/ml (1 ml DMSO stock). For i.p. injection in mice (~30 g, ≤15...

4 weeks ago 659 1 View

How to reduce non-specific bands when detecting CPT1A in mouse liver lysates by Western blot?

Hello, has anyone worked with CPT1A (HY-P81167) in mouse liver tissue using RIPA buffer? I added broad-spectrum protease and phosphatase inhibitors and boiled the lysates at 100 °C for 10 min,...

4 weeks ago 7,801 1 View

Has anyone worked with MOMIPP (HY-119624) and found a solvent that prevents precipitation?

Hi all, I’m working with MOMIPP (Cat. No.: HY-119624, MedChemExpress) and have encountered some solubility issues that I’d like advice on. For my stock, I prepared a 1 mM solution (5 mg fully...

4 weeks ago 8,436 1 View

What is the most suitable method to assess and compare the binding activity of our CD5-Fab antibody to CD5 protein?

Hi everyone, I would like to evaluate the binding activity of our CD5-Fab antibody with CD5 protein and also compare binding activity across different batches of CD5-Fab. Which method would be...

4 weeks ago 8,377 2 View

How can I optimize c-fos IHC in rat uterus to reduce non-specific background while still retaining a clear detectable signal?

Hi all, I’m working on immunohistochemistry for c-fos in rat uterus (1:75). In previous trials with 1:50 and EDTA retrieval, I saw strong non-specific binding. Following advice, I switched to...

4 weeks ago 8,415 2 View

Why doesn’t GSK963 show inhibitory effects on MLKL and RIPK3 expression in my WB experiment?

Hi everyone, I plated HeLa cells at 2 × 10⁵ cells/mL and treated them with or without GSK963 (100 nM). For apoptosis induction, I used 50 or 100 ng/mL TNFα + 1 μM birinapant. For necroptosis...

5 weeks ago 5,931 1 View

What is the appropriate dissolution method for Sacituzumab govitecan (an ADC)?

Hi everyone, I saw on the product website that water is listed as the solvent for this antibody–drug conjugate - Sacituzumab govitecan (HY-132254). Could anyone confirm the recommended procedure...

5 weeks ago 542 1 View

How should I reconstitute Wheat Germ Agglutinin–AF488 (WGA-AF488) and set up stocks for tissue staining?

Hi everyone, I’m planning to prepare a WGA-AF488 (HY-NP163A) stock. What is the appropriate solvent for reconstitution, and the volume to add in order to achieve the recommended stock...

last month 2,417 6 View

What is the solubility protocol of GSK805 in DMSO, PEG300, and Tween-80?

Hi all, I would like to know the solubility of GSK805 in 10% DMSO, PEG300, and Tween-80 with saline, since this is considered a safe vehicle formulation for intratracheal administration to mice....

last month 8,333 3 View

How can I reliably observe doxorubicin fluorescence in AGS tumor cells—incubation method, dose, or timing?

Hi everyone, I recently purchased Doxorubicin hydrochloride (HY-15142) to study drug transport between tumor cells, as I need to observe its fluorescence. I am wondering if the main goal is to...

last month 4,366 1 View

How can I improve the reliability of my GSH/GSSG standard curves, and should I run both assays on the same plate or separately?

Hi, I am preparing to repeat the experiment using GSH and GSSG standards in order to obtain a more reliable standard curve using the GSH/GSSG Assay Kit from MedChemExpress (HY-K0311), particularly...

last month 5,153 2 View

When using Protein A/G Magnetic Beads to bind protein samples, is it normal for the magnetic beads to aggregate?

Hi all, I am using Protein A/G Magnetic Beads (MedChemExpress, HY-K0202) for binding protein samples, and I’ve noticed that the beads tend to aggregate or clump together during the process. Is...

last month 3,002 3 View

Rituximab from CHO cells: does it contain glycans, and how is CoA molecular weight determined?

Hi everyone, I am working with Rituximab expressed in CHO cells and would like to clarify two points. First, does this monoclonal antibody contain glycans due to CHO cell expression? Second, in...

last month 9,841 1 View

How to dissolve gondoic acid in DMSO, and how do I confirm it is truly solubilized rather than just dispersed?

Hi all, Does anyone know of a detailed method to dissolve gondoic acid in DMSO? Since both gondoic acid and DMSO are transparent liquids, how can I determine whether gondoic acid is fully...

last month 6,896 1 View

What are some practical tips to improve the detection of BCL-2 protein by western blotting?

Hello everyone, I am working with samples derived from mice and rat models, and I’ve been having difficulty detecting BCL-2 (Cat.No: HY-P80565) consistently by western blotting. The signal often...

last month 2,943 1 View

Can Cyclo(RGDyK) function as an αVβ5 inhibitor, and is water a suitable solvent for in vivo mouse studies?

Hi all, I have two questions regarding Cyclo(RGDyK): Can Cyclo(RGDyK) be used as a functional inhibitor of the vitronectin receptor (αVβ5)? Since Cyclo(RGDyK) can be dissolved in either water or...

last month 4,785 1 View

Is the following administration plan for AMD3100 and CTCE-0214 feasible in Wistar rat (200 g) model experiment?

Hi everyone, I am planning an animal experiment using male Wistar rats, with grouping and interventions already designed. I have consulted the literature to determine the dosage for all reagents,...

last month 2,695 1 View

Glucagon stimulation and p-PKA expression in primary mouse hepatocytes: what are the recommended concentrations and treatment times?

Hi all, I am currently investigating the effects of glucagon signaling on PKA activation in hepatocytes with Glucagon (1-29), bovine, human hydrochloride (HY-P0082A). Specifically, I would like to...

last month 8,254 1 View

What is the most suitable material for storing a biosimilar Bevacizumab solution to ensure stability and prevent loss of activity?

Hi all, this is my first time working with a biosimilar form of Bevacizumab (HY-P9906 from MedChemExpress), and I want to ensure that the storage conditions are appropriate. Specifically, I am...

last month 5,128 3 View

What is the recommended protein concentration for immobilization with Ni-NTA Magnetic Agarose beads and protocol set up for ELISA?

Hi all, I recently purchased Ni-NTA Magnetic Agarose beads (from MedChemExpress, HY-K0241) and would like to immobilize a His-tagged protein. After immobilization, I plan to perform an ELISA to...

last month 9,589 1 View

How to use Pacritinib in an in vivo mouse model?

Hi everyone, I am planning to use Pacritinib (MedChemExpress, HY-16379) in an in vivo mouse model, and have some enquiries as follow: Is this product suitable for intraperitoneal (i.p.)...

last month 1,942 1 View

How to identify hypoxic areas using pimonidazole in vivo?

Hi all, I plan to inject pimonidazole (MedChemExpress, HY-105129A) intravenously into mice, then collect tissues, fix them in formalin, and prepare paraffin-embedded sections. However, I’m unsure...

last month 2,452 1 View

How to improve the solubility of Atrasentan?

Hi everyone, I am dissolving Atrasentan (HY-15403) in DMSO, PEG 300, Tween 80, and saline, followed by mixing with ddH2O for oral administration. According to the literature, I plan to use this...

last month 868 1 View

How can I prepare GSK126 for in vivo dosing in BALB/c Nude mice xenograft experiments?

Hi all, I purchased 25 mg of GSK126 (HY-13470) for combination treatment in BALB/c Nude mice (A549 xenograft). Literature shows various dosages ranging from 50 mg/kg to 300 mg/kg for different...

last month 3,664 2 View

Why are the bands weak and at the wrong position when using a Cleaved-Caspase 8 antibody in Western blot?

Hi everyone, I’m using Cleaved-Caspase 8 Antibody (YA792) (HY-P80624 from MedChemExpress) in MCF7 cells to perform a Western blot after heat stress treatment. However, I’m running into a few...

05 August 2025 4,873 1 View

What is the solubility and best dissolution method for UNC569?

Hi everyone, I am currently working with UNC569 (HY-117596) but I am unsure about its solubility. Could you please advise which solvent works best for dissolving it? Also, if you have any...

04 August 2025 2,415 2 View

Why does NF-κB p65 show multiple bands or increased molecular weight in Western blot?

Hi everyone, I’ve been working with NF-κB p65 (HY-P80765) in Western blot (WB) experiments, but I’m consistently observing multiple bands or an increased molecular weight for p65. This is...

01 August 2025 8,216 1 View

No NLRP3 bands detected in Ishikawa and HEC-1-A cells using YA266 antibody—lack of expression or other issue?

Hello everyone, I used the NLRP3 Antibody (YA266, HY-P80246) to detect NLRP3 protein in Ishikawa and HEC-1-A cell lines via western blot, but I wasn’t able to detect any specific bands. Does this...

31 July 2025 2,917 1 View

Is it normal that SC79 turned pink after being prepared as a stock solution with DMSO?

Hi everyone, I recently prepared a stock solution of SC79 from Medchemexpress (HY-18749) using DMSO as the solvent. After dissolving, I noticed that the solution developed a pink coloration...

30 July 2025 1,184 2 View

How should insulin powder be dissolved and stored for intraperitoneal injection?

Hi everyone, I ordered insulin from MCE (HY-P0035) for use in animal experiments via intraperitoneal injection, and the working concentration I need is 0.003 mg/mL. I’m unsure about the best...

29 July 2025 1,146 2 View

To perform an immunofluorescence staining on expanded primary/stem cells, what are the steps for processing and staining?

Hi all, I’m working with expanded primary/stem cells and I need to perform immunofluorescence staining to assess the expression of specific markers. Could anyone provide a step-by-step protocol or...

28 July 2025 6,142 1 View

How to use the membrane-permeable fluorescent probe Laurdan?

Hi everyone, I recently performed Laurdan staining on live cells using a 10 µM working solution diluted in complete culture medium using Laurden from MCE (HY-D0080). I incubated the cells at 37°C...

25 July 2025 7,242 1 View

Can someone recommend a strong adherent cell dye?

Hi everyone, Our lab is planning an immune co-culture experiment involving both adherent and suspension cells. We’ll be using a high-throughput imaging system to monitor the state of the adherent...

23 July 2025 346 3 View

What is the expected protein yield from mouse liver?

Hi everyone, I’m currently extracting total protein from mouse liver tissue using RIPA buffer supplemented with a protease inhibitor cocktail. After quantifying the protein concentration using...

22 July 2025 754 4 View

Why is the recovery rate of the linearized vector low after I have done the restriction digestion?

Hi all, I used the EcoR I endonuclease I just bought from Medchemexpress (HY-KE7014), and the results after recovery are as shown in the screenshot. The first four lanes are 20ul of linearized...

21 July 2025 6,587 8 View

Conjugation of PolyConjugation of Polyclonal Antibodies to Magnetic Beads: Best Approach?clonal Antibodies to Magnetic Beads: Best Approach?

Hi everyone, I'm looking for a reliable protocol to conjugate polyclonal antibodies to magnetic beads for isolating target proteins from serum. Given the wide variety of magnetic bead types on...

18 July 2025 5,182 1 View

Does anyone know the answers to the following questions about cell oxidatioin staining kit?

I bought a cell oxidation staining kit from MedChemExpress (HY-K1089) and would like to ask: Can I use plates other than 6-well plates for staining, such as 12-well plates, 24-well plates, and...

17 July 2025 8,276 3 View

Is there a protocol for subcutaneous injection of Allo-aca to induce modeling?

Hi all, I am currently working on animal model construction and plan to use Allo-aca from Medchemexpress (HY-P3212) to induce the model by subcutaneous injection for 21 consecutive days. I am...

16 July 2025 4,964 1 View

Can PGSK diacetate (5/6-mixture) be used to detect ferroptosis in a high-content format in a 96-well plate?

Hi everyone, Recently, I’ve been considering using a high-content screening (HCS) platform to detect ferroptosis-related changes, and I'm planning to process samples in 96-well plates to improve...

15 July 2025 645 1 View

There are black spots in the middle of the RAW 264.7 macrophages I cultured. What should I do?

Hi all, I observed black spots in my cultured macrophages. The complete culture medium I used includes DMEM, FBS, penicillin-streptomycin, and amphotericin B. However, the cells still got...

14 July 2025 9,820 1 View

Can someone recommend a suitable Western Blot stripping buffer for removing primary and secondary antibodies from PVDF membranes?

Hi, The Western Blot stripping buffer I used previously did not effectively remove all antibody staining despite following the instructions and incubating the membrane in the buffer for up to 6...

11 July 2025 2,038 2 View

I bought NLRP3 antibody for immunohistochemistry, but I couldn't detect any signal. Why is this happening?

Hi everyone, I recently purchased an NLRP3 antibody for immunohistochemistry (IHC) from Medchemexpress (HY-P80246), but unfortunately, I haven’t been able to detect any signal in my tissue...

09 July 2025 1,496 7 View

Is fish gelatin blocking buffer suitable for biotin-streptavidin color development system?

Hi all, I’m considering using the rapid blocking buffer power (HY-K1027) in my experiment, but I’m not sure if it’s fully compatible with the biotin-streptavidin system. Does fish gelatin contain...

08 July 2025 2,038 0 View

My cells are not in good condition after PolyFast transfection. What's going on?

Hi, I’ve been using PolyFast transfection reagent from Medchemexpress (HY-K1014) and followed the general protocol. However I’ve noticed that my cells don’t look healthy afterward and I'm...

04 July 2025 1,394 6 View

What are the inhibitors that inhibit EHD2 from entering the nucleus and inhibiting the internalization of caveolae on the cell membrane?

Hello, I’m studying the role of EHD2 in regulating caveolae dynamics, particularly its function in stabilizing caveolae at the plasma membrane. I’m interested in knowing: What are the known...

03 July 2025 2,123 1 View

The storage instruction for Angiotensin II suggests to store in liquid nitrogen, what does this mean and how shall I do this?

Hi all, I recently purchased Angiotensin II from Medchemexpress (HY-13948) for my experiments, and the storage instructions from the supplier mention that it should be stored in liquid nitrogen....

03 July 2025 1,282 3 View

Can JC-1 (CBIC2) be used for tissue section staining and how does it work?

Hi everyone, I'm currently working on a project involving mitochondrial membrane potential, and I’ve come across the dye JC-1 (CBIC2) from Medchemexpress (HY-15534). I am wondering whether it...

02 July 2025 6,267 1 View