For reconstituting your WGA-AF488 (HY-NP163A), the appropriate solvent is anhydrous dimethyl sulfoxide (DMSO). This solvent ensures the lyophilized powder dissolves completely and helps maintain the stability and fluorescent activity of the conjugate. Using water or buffer is not recommended, as it can lead to aggregation and precipitation of the product.

To prepare the stock solution, a concentration of 5-10 mg/mL is standard. For a common 5 mg vial, add 500 µL of DMSO to achieve a final concentration of 10 mg/mL. This creates a concentrated stock where each microliter contains 10 micrograms of the WGA-AF488 conjugate, which is ideal for subsequent dilutions into aqueous buffers for your experiments.

Once reconstituted, it is crucial to aliquot the solution into single-use volumes to avoid repeated freeze-thaw cycles. These aliquots must be stored at -20 °C or lower and protected from light to preserve the integrity of the fluorescent dye. This process ensures maximum stability and performance for your cell staining applications.

You can prepare a 1 mg/mL stock solution with water, then centrifuge and use the supernatant. (Centrifugation is performed to remove protein aggregates in the solution, thereby reducing nonspecific binding.)

Note: The 1 mg/mL stock solution prepared in water can be stored at 2–8°C for up to one week, or aliquoted and stored at –20°C for up to six months. Keep protected from light.

The answer to this question is provided by MedChemExpress Technical Support. [email protected]

Hi Anthony Ayodeji Adegoke , thanks a lot for your input! However, there is a chance that DMSO may denature the lectin component in WGA. Therefore, I will go with water as the solvent to prepare the stock solution.

@fan Janice , I understand the concern, but when you dilute the DMSO into the aqueous protein solution at the final DMSO concentration ≤0.1–0.5%, it cannot destabilise the lectin.

Best wishes

Anthony Ayodeji Adegoke Thank you, I’ll keep it in mind!

You can use the following online tool to calculate this:

https://www.alfa-chemistry.com/solution-dilution-calculator.htm

https://www.alfa-chemistry.com/mass-molarity-calculator.htm