Hi all, I plan to inject pimonidazole (MedChemExpress, HY-105129A) intravenously into mice, then collect tissues, fix them in formalin, and prepare paraffin-embedded sections. However, I’m unsure about the best way to identify pimonidazole signals in the tissue slides. Should I use a specific staining protocol or antibody for detection? Any recommendations or tips for optimizing the identification of hypoxic regions would be greatly appreciated.
Pimonidazole is a 2-nitroimidazole that is specifically reduced and activated in hypoxic cells, forming stable conjugates with sulfhydryl groups in proteins, peptides, and amino acids. Subsequently, antibodies need to be tested. However, we regret to inform you that we haven't launched the relevant antibodies at this time.
The biological activities of Pimonidazole hydrochloride (HY-105129) and Pimonidazole (HY-105129A) are equivalent. Among them, Pimonidazole hydrochloride is in the free state, while Pimonidazole is in the salt form, which provides better solubility in water. Therefore, it is recommended to prioritize the use of Pimonidazole. The introduction to its usage in vivo can be found on our website. The answer to this question comes from MedChemExpress Technical Support.
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