Hi all, I am using Protein A/G Magnetic Beads (MedChemExpress, HY-K0202) for binding protein samples, and I’ve noticed that the beads tend to aggregate or clump together during the process. Is this kind of aggregation a normal phenomenon when working with magnetic beads, or does it indicate an issue with the beads, buffer conditions, or handling technique? Thanks in advance for any insights!
According to our Protein A/G Magnetic Beads Manual, It is normal for magnetic beads to aggregate when combined with antibodies and it does not affect the activity.
The answer to this question is provided by MedChemExpress Technical Support. [email protected]
I've been using anti-HA magnetic beads and these regularly clump during incubation with a protein mixture. The clumps are quite easy to dissociate with mild pipetting.
Once I tried purifying an easily aggregating protein with a similar kind of beads and I noticed the beads were even more clumpy then usual. It seemed I was getting low yields because the protein of interest aggregated on my beads when I washed away all the other proteins.
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