Glucagon stimulation and p-PKA expression in primary mouse hepatocytes: what are the recommended concentrations and treatment times?

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Hi all, I am currently investigating the effects of glucagon signaling on PKA activation in hepatocytes with Glucagon (1-29), bovine, human hydrochloride (HY-P0082A). Specifically, I would like to know whether glucagon treatment has been shown to increase phosphorylated PKA (p-PKA) levels in primary mouse hepatocytes, and what ranges of concentrations and incubation times is recommended. Thank you in advance!

Lucian Miles

Hi Fan,

In this paper using mouse primary hepatocytes, 10 nM glucagon for 10 minutes was shown to increase p-PKA expression. PMID: 33030802 (see figure)
I also checked another paper, also in primary hepatocytes, where glucagon (100 nM, 5 minutes) was shown to increase p-PKA expression. PMID: 36243006

The answer to this question is provided by MedChemExpress Technical Support.

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