Glucagon stimulation and p-PKA expression in primary mouse hepatocytes: what are the recommended concentrations and treatment times?

19 August 2025 1 8K Report

Hi all, I am currently investigating the effects of glucagon signaling on PKA activation in hepatocytes with Glucagon (1-29), bovine, human hydrochloride (HY-P0082A). Specifically, I would like to know whether glucagon treatment has been shown to increase phosphorylated PKA (p-PKA) levels in primary mouse hepatocytes, and what ranges of concentrations and incubation times is recommended. Thank you in advance!

Lucian Miles

Hi Fan,

In this paper using mouse primary hepatocytes, 10 nM glucagon for 10 minutes was shown to increase p-PKA expression. PMID: 33030802 (see figure)
I also checked another paper, also in primary hepatocytes, where glucagon (100 nM, 5 minutes) was shown to increase p-PKA expression. PMID: 36243006

The answer to this question is provided by MedChemExpress Technical Support.

[email protected]

Badges
Science topic

More Fan Janice's questions See All

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Integral Membrane Protein Purification Help?

Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...

30 May 2024 7,604 3 View

If anyone has experience using Silvaco for simulating 2D memory?

Hello everyone, I'm curious to know if anyone has experience using Silvaco for simulating 2D memory. I'm particularly interested in exploring the memory characteristics of two-dimensional...

14 May 2024 7,781 0 View

Is there any automated catwalk Hydraulic Tray - （catwalks）ajustable resource?

...

10 May 2024 8,189 0 View

How to reverse the direction of two same promoters in the construction of plasmid?

Hello！ When constructing the plasmid, I used the same promoter. However, the direction of each expression cassette promoter in the obtained plasmid is the same, and the plasmid with the opposite...

07 April 2024 9,022 1 View

How should I test the moderating effect of information sharing motivation?

If I wanted to experimentally investigate the effect of information quality on rumour sharing intentions and the moderating role of information sharing motivation in this (we would manipulate...

24 March 2024 6,428 8 View

Laplace Transform for Approximating Nonlinear PDE Solutions?

Is anyone interested in or looking for further collaboration on approximating the solution of nonlinear PDEs (of the diffusion-type) using the Laplace transform? In essence, its derived results...

19 March 2024 4,713 3 View

How do I find a copy of a particular paper that was published in 2017 about the discovery of a new meson?

the authors were mostly from the University of Michigan, and it was published in the summer of 2018

16 January 2024 9,568 3 View

Rheometer testing resin and prepreg: Flow mode or Oscillation mode?

Hi everyone. Greetings to you all. Recently, I tested the viscosity of the epoxy resin and CF/epoxy prepreg samples using a TA-discoveryHR-1 rheometer. Firstly, in an isothermal way(holding...

19 December 2023 5,798 2 View

Can anyone share this literature "How to score and interpret single-item health status measures: a manual for users of the SF-8™ Health Survey"?

How to score and interpret single-item health status measures: a manual for users of the SF-8™ Health Survey

01 December 2023 2,371 0 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View