Hi all, I recently purchased Ni-NTA Magnetic Agarose beads (from MedChemExpress, HY-K0241) and would like to immobilize a His-tagged protein. After immobilization, I plan to perform an ELISA to verify successful binding, followed by further applications involving oligonucleotides that interact with the protein.
To ensure the best performance and avoid extensive optimization, I would really appreciate any information shared regarding:
• Suggested protein concentration for immobilization
• Any relevant protocols or tips for ELISA setup with these beads
Thank you in advance for your support.
1. The loading capacity of Ni-NTA Magnetic Agarose beads (Cat. : HY-K0241) is as follows. The protein concentration of the customer can be used as a reference, or it can be slightly higher.
2. For specific experiments (eg: ELISA), please refer to the literature reports: PMID: 16642995
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