Hi all, I need to prepare a gavage solution at 10 mg/mL (dose: 100 mg/kg, 200 μL for a 20 g mouse). Could you recommend the best solvent system or dissolution method? Thank you so much in advance!
We did not find a dissolution protocol for this dose in the literature. The measured formulation is as follows: 1 mg of the compound dissolved in 100 μL of 0.5% CMC-Na/saline water, with the addition of 5 μL of Tween-80 (concentration: 10 mg/mL). This mixture achieves a suspension state. For animal administration via intraperitoneal or oral gavage, this suspension is suitable as long as it is uniform and does not clog the needle. However, it has been observed that precipitation occurs after 5 minutes of standing. Administration should be completed before precipitation forms.
The preparation method for 0.5% CMC-Na/saline water is as follows:
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