Hi everyone, I’ve reviewed several studies on OGT2115 and noticed considerable variation in the solvent compositions used. Could anyone share guidance on the recommended formulation—specifically the appropriate percentages of DMSO, PEG400, Tween-80, and saline—for preparing OGT2115 for oral gavage or intraperitoneal injection in mice? Any established protocols or practical tips would be greatly appreciated.
Hi,
Generally speaking, for normal mice, the commonly used ratio is 10% DMSO+40% PEG300+5% Tween-80+45% Saline. For weak mice such as those with immune deficiency or gene elimination, it is recommended to reduce the proportion of DMSO : 2% DMSO+40% PEG300+5% Tween-80+53% Saline. Both clear or uniform suspensions can be used for intraperitoneal or oral gavage administration.
However, for the OGT2115 (HY-100898) product, we tested that this scheme is precipitated and is not recommended. If you have a low dose (≤1.14 mg/mL) and a small number of dosings （≤ half a month）, you can consider Protocol 1: 10% DMSO+ 90% Corn Oil. If you have a high dose, you can consider the 0.5% CMC-Na/saline water ready-to-use scheme. Both clear or uniform suspensions can be used for intraperitoneal or intragastric administration.
The answer to this question is provided by MedChemExpress Technical Support. [email protected]
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