Hello everyone,
I used the NLRP3 Antibody (YA266, HY-P80246) to detect NLRP3 protein in Ishikawa and HEC-1-A cell lines via western blot, but I wasn’t able to detect any specific bands.
Does this result necessarily indicate that these endometrial cancer cell lines do not express NLRP3, or could it be due to antibody sensitivity, low protein abundance, or protocol-related issues (e.g., lysis buffer, loading amount, or detection conditions)?
Has anyone successfully detected NLRP3 in these or similar cell lines? Any tips or reference data would be very helpful to clarify whether this is a true negative expression or a technical limitation.
Thank you so much in advance!
NLRP3 is an inflammation-related protein, and under normal cellular or tissue conditions, the expression level of inflammatory factors is relatively low. Typically, their expression is assessed after inflammatory model induction.
Your samples are human endometrial carcinoma cell lines, Ishikawa and HEC-1-A, and some references have indeed confirmed that NLRP3 is expressed in these cells. The experimental conditions you described do not appear to have any issues.
It may also be useful to include a positive control sample in your experiment to rule out potential problems with the antibody.
The answer to this question comes from MedChemExpress (MCE) Technical Support. [email protected]
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