Regarding the use of Cleaved-Caspase 8 Antibody (YA792) (HY-P80624) for WB:

1. Caspase 8 (Q14790_HUMAN) plays a central role during the execution phase of apoptosis. It is expressed in nine isoforms through alternative splicing, with molecular weights ranging from 26 kDa to 62 kDa. The most common isoform, Q14790-1 Alpha-1, consists of 479 amino acids and has a predicted molecular weight of ~55 kDa (reference: UniProt https://www.uniprot.org/uniprotkb/Q14790/entry#sequences).

2. Bands around 34 kDa—similar to the one you observed—have been reported in published papers. For example, see PMID: 28846113.

3. In our own WB validation using HY-P80624, we typically observed a band at ~43 kDa, as shown in the attached image.

4. You can assess whether your current band corresponds to the target protein by comparing its signal pattern and trend under different sample treatments. If the trend is unclear, consider adding:

• A negative control (a sample with knockdown or knockout of the target protein, or known to have low expression), or
• A positive control (an overexpression sample), and use these to confirm whether the band is specific.

5. For sample treatment, there are reports where heat shock and staurosporine treatment were used to induce cleavage. You might want to refer to those conditions for comparison.

The answer to this question comes from MedChemExpress Technical Support.

[email protected]