Hi everyone, I recently purchased Doxorubicin hydrochloride (HY-15142) to study drug transport between tumor cells, as I need to observe its fluorescence. I am wondering if the main goal is to detect fluorescence, how should I incubate the tumor cells?

I came across some literature suggesting suspension incubation for about 8 minutes before plating the cells. However, in my experiments with AGS gastric cancer cells, I tested 1 µM, 500 nM, and 250 nM for 24 h, but I could not detect any red/green fluorescence. Should I consider using a different incubation method, or would adjusting the incubation time and concentration be more effective? Thank you so much in advance!