Hi everyone, I am working with Rituximab expressed in CHO cells and would like to clarify two points. First, does this monoclonal antibody contain glycans due to CHO cell expression? Second, in the Certificate of Analysis (CoA), how is the molecular weight typically determined—is it based on intact mass analysis or after enzymatic digestion? Thank you in advance!
Hi，
Rituximab is expressed in CHO cells and contains glycosylation modifications. The molecular weight provided in the Certificate of Analysis (CoA) is calculated based on the amino acid sequence of the antibody and represents the theoretical molecular weight, which does not include post-translational modifications such as glycosylation.
The answer to this question is provided by MedChemExpress Technical Support.
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