Hi everyone,
I recently prepared a stock solution of SC79 from Medchemexpress (HY-18749) using DMSO as the solvent. After dissolving, I noticed that the solution developed a pink coloration instead of remaining clear.
Is this a normal characteristic of SC79, or could it indicate degradation, contamination, or a reaction with the solvent? The compound was freshly opened and handled under standard lab conditions.
Has anyone else observed this color change with SC79 solutions, and do you know if it affects the stability or activity of the compound? Any advice would be greatly appreciated!
After SC79 (HY-18749) is prepared into a DMSO stock solution, a pink color is an abnormal phenomenon. SC79 in solution is unstable, and turning pink may be a sign of oxidative degradation of the compound. Based on chemical stability considerations, it is recommended to stop using the discolored solution. To ensure experimental reliability, we recommend: 1) Aliquot the stock solution immediately after preparation; 2) Maintain storage conditions at -80℃; 3) Check the solution's state before each use. If there is a color change (e.g., turning pink) or reduced activity, discard it and prepare a new solution. These measures can maximize the stability of the compound.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
A pink coloration of SC79 in DMSO is an abnormal phenomenon and indicates that the compound has undergone oxidative degradation
. SC79 is known to be unstable in a solution state, so the color change suggests a loss of potency and means the solution should be discarded.
Handling and stability recommendations for SC79
Based on guidance from MedChemExpress (MCE) technical support and other researchers who have experienced this issue, here are the best practices for preparing and storing your SC79 stock solution:
- Aliquot immediately: After preparing a stock solution in DMSO, immediately divide it into smaller, single-use aliquots. This minimizes repeated exposure of the full stock to air and temperature changes.
- Store at -80°C: Store the aliquots in a -80°C freezer to maximize stability and prevent degradation. SC79 is unstable in solution at higher temperatures.
- Use freshly: For experiments, thaw an aliquot and use it immediately. Do not store the thawed solution or subject it to multiple freeze-thaw cycles, as this can cause degradation.
- Check before use: Always check the aliquot for any discoloration before use. If you see a pink color or any other change, discard it and use a fresh aliquot.
This protocol minimizes the risk of oxidative degradation and ensures the reliability of your experiments by preserving the compound's stability and activity.
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