Hello, has anyone worked with CPT1A (HY-P81167) in mouse liver tissue using RIPA buffer? I added broad-spectrum protease and phosphatase inhibitors and boiled the lysates at 100 °C for 10 min, followed by reducing SDS-PAGE, but I keep getting many non-specific bands on the blot. Does anyone have experience optimizing this or suggestions for improving the specificity? Thank you so much in advance!
Hi，
This antibody is polyclonal and may exhibit some artifacts. Since your sample is tissue, which is likely more complex than cells, you can optimize the following aspects and adjust your experiment.
1. Reduce the protein loading amount.
2. Reduce the antibody concentration, shorten the incubation time, and optimize the primary and secondary antibody concentrations.
The answer to this question is provided by MedChemExpress Technical Support.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Publication