Hi everyone, I am doing an experiment involving MSAB (HY-120697) and according to the datasheet, MSAB dissolves well in DMSO at 10 mg/ml (1 ml DMSO stock). For i.p. injection in mice (~30 g, ≤15 days treatment), the plan was to dilute the stock into a 10% DMSO + 90% corn oil vehicle to achieve ~1 mg/ml per mouse. However, mixing DMSO with corn oil results in phase separation, although this issue is not mentioned in the instructions. What practical strategies can be used to prepare a homogeneous vehicle for MSAB i.p. delivery? Would switching to a PEG300 + Tween80 solubilization system ensure miscibility and stable formulation? Any insights would be greatly appreciated!
Hello Fan,
Yes, you are right. By adding PEG300 and Tween80 you can prevent phase separation between corn oil and DMSO.
Tween80 is a nonionic surfactant with a hydrophilic head and a hydrophobic tail. When mixed with corn oil and a polar solvent like DMSO, the hydrophobic tails will interact with corn oil and the hydrophilic heads will interact with the polar solvent, forming a stable emulsion.
PEG300 is a hydrophilic, polymeric liquid. It is commonly used as a co-solvent and solubilizer. In this case, PEG300 will help to increase the solubility of both the corn oil and DMSO in the mixture. It can also help stabilize the emulsion created by Tween80.
By combining these two substances, you are leveraging Tween80's ability to emulsify and PEG300's ability to enhance solubility.
Best,
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies