Hi everyone, I am planning an animal experiment using male Wistar rats, with grouping and interventions already designed. I have consulted the literature to determine the dosage for all reagents, but I would like help with the following points:
- AMD3100: 1.34 mg/kg, concentration: 0.268 mg/mL
- CTCE-0214: 12.5 mg/kg
I'd really appreciate any insight or suggestion, thanks in advance!
For rats weighing 200-300 g, the injection volume should generally not be as high as 1 mL. Instead, the commonly recommended range is 200–400 μL. This helps minimize stress and ensures better tolerability in the animals during repeated dosing.
If you intend to inject 1 mL, the final working concentration should be 1.34 mg/kg*0.2 kg/1mL=0.268 mg/ml. It's reasonable. You can also calculate the working cencentration for CTCE-0214 in a same way.
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