Hi all,
I recently purchased Angiotensin II from Medchemexpress (HY-13948) for my experiments, and the storage instructions from the supplier mention that it should be stored in liquid nitrogen. I’m a bit confused about what exactly that means in practice. Most of the reagents I use don’t have such extreme storage requirements, so I want to make sure I’m handling this correctly.
Thank you so much in advance!
Fan Janice ,
Angiotensin II is relatively active, so it needs to be stored in an inert gas. The usual storage method is to store it in nitrogen.
The steps for inert gas replacement are as follows:
1. Put an argon/nitrogen balloon on the tail of the syringe, replace the air in the syringe first, and then install the needle (the joint between the balloon mouth and the syringe can be wrapped with a sealing film to ensure airtightness).
2. Insert the needle into the bottle (tube) where the gas needs to be replaced. Avoid facing the sample in the direction of the needle to prevent the powder from being blown up. There is no need to squeeze the balloon by hand during the operation. The gas replacement time can be calculated according to the flow rate (the natural gas flow rate of the 0.6 mm needle device is greater than 0.2 mL/second). Be careful not to blow up the powder; after the operation is completed, the balloon needs to be sealed for next use.
3. If the sample needs to be packaged, the gas in the empty tube should be replaced first, and then the gas replacement should be performed again after the sample is added.
The answer to this question comes from MedChemExpress (MCE) Technical Support.
The storage advice is to submerge in liquid N2 in a Dewar.
Otherwise can you use a minus 80 freezer?
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