PGSK diacetate (5/6-mixture) (HY-126823, MCE) is a fluorescent heavy metal ion indicator that can specifically bind to various divalent metal ions (including Fe²⁺, i.e., ferrous iron) within cells, making it theoretically suitable for detecting ferroptosis. Reference method (PMID: 37979586): Cell treatment: Seed the cells to be tested (e.g., glioblastoma stem cells, GSCs) in a culture plate and perform relevant treatments (e.g., gene knockout, drug treatment, etc.) to induce or inhibit ferroptosis. Dye incubation: Add PGSK diacetate dye to the cells and incubate at 37°C for 30 minutes to allow the dye to fully enter the cells and bind to free Fe²⁺. Fluorescence detection: Detect intracellular fluorescence signals using immunofluorescence microscopy or flow cytometry. If ferroptosis occurs, the accumulation of free Fe²⁺ will cause characteristic changes in PGSK fluorescence signals (e.g., the "loss of immunofluorescence staining" mentioned in the literature, i.e., fluorescence signal weakening or disappearance, possibly due to fluorescence quenching upon Fe²⁺ binding). The answer to this question comes from MedChemExpress (MCE) Technical Support. [email protected]